We partner with the two other IGS core facilities to provide a full range of services. The Genomics Resource Center (GRC) is our sequencing partner for amplicon libraries. The Informatics Resource Center (IRC) provides analytical and computing infrastructure for data analysis. We package our services together to provide a seamless, high quality product for microbiome projects.
The MSL routinely processes samples from all over the world from many different sample types, including human, animal, and environmental sources.
MSL Workflow Overview
Mobio PowerMicrobiome DNA/RNA
Hamilton STAR 4x96 preps/day
Gene specific primer (V3V4 etc) KAPA HiFi polymerase 20-25 cycles
TTP Mosquito HV 20+ plates/day
Complementary extension primer set Dual indexing 10-12 cycles
TTP Mosquito HV 20+ plates/day
Amplicons normalized within PCR plate & across all pooled plates
Hamilton STARlet
HiSeq 2500 RR 2 channels per flowcell
4 day run time ~8x throughput of MiSeq
1584 samples per channel
dada2 based workflow
Initial analysis of run metrics
Analysis of run controls
Split by project
The GRC and MSL have co-developed a high-throughput sequencing solution in partnership with Illumina that makes use of HiSeq Rapid Run sequencing chemistry to produce 300 bp paired-end high quality sequence data.